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KMID : 0380020100250050471
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 5 p.471 ~ p.477
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Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes
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Kang Hee-Jeong
Li Hong-Xian
Kim Yong-Jin
Kim Seong-Jun
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Abstract
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In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 h-1), 6 h (0.07 h-1), 3 h (0.13 h-1). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 h-1.
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KEYWORD
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Ethanol, productivity, batch, high-cell-density, SSF, fill & draw, continuous culture, food wastes
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